3' splice site selection in dicot plant nuclei is position dependent.

Intronic and exonic sequences modulate 5' splice site selection in plant nuclei.

Pre-mRNA transcripts in a variety of organisms, including plants, Drosophila and Caenorhabditis elegans, contain introns which are significantly richer in adenosine and uridine residues than their flanking exons. Previous analyses using exonic and intronic replacements between two nonequivalent 5'splice sites in the 469 nt long rbcS3A intron 1 provided the first evidence indicating that, in bot...

Splice site selection in the dystrophin gene

The dystrophin gene is the largest gene in the human genome, and it codes for a protein which plays an important role in anchoring the cytoskeleton to the cell membrane. Mutations in the gene may cause Duchenne muscular dystrophy (DMD) or Becker muscular dystrophy (BMD), an allelic variant. Currently genetic therapy holds great promise in finding a cure through exon skipping by restoring the pr...

SRp30c is a repressor of 3' splice site utilization.

Several intron elements influence exon 7B skipping in the mammalian hnRNP A1 pre-mRNA. We have shown previously that the 38-nucleotide CE9 element located in the intron separating alternative exon 7B from exon 8 can repress the use of a downstream 3' splice site. The ability of CE9 to act on heterologous substrates, combined with the results of competition and gel shift assays, indicates that t...

Feature subset selection for splice site prediction

MOTIVATION The large amount of available annotated Arabidopsis thaliana sequences allows the induction of splice site prediction models with supervised learning algorithms (see Haussler (1998) for a review and references). These algorithms need information sources or features from which the models can be computed. For splice site prediction, the features we consider in this study are the presen...

A Novel Splice Site Mutation in HPS1 Gene is Associated with Hermansky-Pudlak Syndrome-1 (HPS1) in an Iranian Family